Over-expression of the poplar expansin gene PtoEXPA12 in tobacco plants enhanced cadmium accumulation.
Identifieur interne : 000D60 ( Main/Exploration ); précédent : 000D59; suivant : 000D61Over-expression of the poplar expansin gene PtoEXPA12 in tobacco plants enhanced cadmium accumulation.
Auteurs : Hao Zhang [République populaire de Chine] ; Yana Ding [République populaire de Chine] ; Junkai Zhi [République populaire de Chine] ; Xiaoyu Li [République populaire de Chine] ; Huabo Liu [République populaire de Chine] ; Jichen Xu [République populaire de Chine]Source :
- International journal of biological macromolecules [ 1879-0003 ] ; 2018.
Descripteurs français
- KwdFr :
- MESH :
- biosynthèse : Protéines végétales.
- génétique : Populus, Protéines végétales, Tabac, Végétaux génétiquement modifiés.
- métabolisme : Cadmium, Tabac, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : Plant Proteins.
- chemical , genetics : Plant Proteins.
- chemical , metabolism : Cadmium.
- genetics : Plants, Genetically Modified, Populus, Tobacco.
- metabolism : Plants, Genetically Modified, Tobacco.
Abstract
As important cell wall proteins in plants, expansins are involved in a serious of abiotic stress resistance such as drought, heat, salt, even heavy metals. To understand the role of expansins in cadmium (Cd) stress, we analyzed the expression patterns of 36 expansin genes in Populus tomentosa. A Cd-induced expansin gene, PtoEXPA12, was identified, cloned, and transformed into tobacco plants. After treatment with Cd, the transgenic plants showed stronger symptoms of Cd toxicity as to the wild-type tobacco plants. Further physiological tests showed that the transformants had higher relative electrolyte leakage and superoxide dismutase activity, more malondialdehyde and H2O2 content, and lower chlorophyll content in Cd stress. Cd content measurement showed it is 1.40-2.07-fold higher and 1.29-1.38-fold higher separately in roots and shoots of transgenic plants than those in wild-type plants, while the transfer coefficient value kept invariably even decreased. Therefore, PtoEXPA12 was really involved in Cd uptake and accumulation, and led to Cd toxicity of cells. It would be a potentially applicable part in phytoremediation system.
DOI: 10.1016/j.ijbiomac.2018.05.053
PubMed: 29758311
Affiliations:
Links toward previous steps (curation, corpus...)
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Electronic address: jcxu282@sina.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Engineering Laboratory of Tree Breeding, Beijing Forestry University, 100083</wicri:regionArea><wicri:noRegion>100083</wicri:noRegion></affiliation></author></analytic><series><title level="j">International journal of biological macromolecules</title><idno type="eISSN">1879-0003</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cadmium (metabolism)</term><term>Plant Proteins (biosynthesis)</term><term>Plant Proteins (genetics)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Populus (genetics)</term><term>Tobacco (genetics)</term><term>Tobacco (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cadmium (métabolisme)</term><term>Populus (génétique)</term><term>Protéines végétales (biosynthèse)</term><term>Protéines végétales (génétique)</term><term>Tabac (génétique)</term><term>Tabac (métabolisme)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cadmium</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants, Genetically Modified</term><term>Populus</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Populus</term><term>Protéines végétales</term><term>Tabac</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cadmium</term><term>Tabac</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">As important cell wall proteins in plants, expansins are involved in a serious of abiotic stress resistance such as drought, heat, salt, even heavy metals. To understand the role of expansins in cadmium (Cd) stress, we analyzed the expression patterns of 36 expansin genes in Populus tomentosa. A Cd-induced expansin gene, PtoEXPA12, was identified, cloned, and transformed into tobacco plants. After treatment with Cd, the transgenic plants showed stronger symptoms of Cd toxicity as to the wild-type tobacco plants. Further physiological tests showed that the transformants had higher relative electrolyte leakage and superoxide dismutase activity, more malondialdehyde and H<sub>2</sub>O<sub>2</sub> content, and lower chlorophyll content in Cd stress. Cd content measurement showed it is 1.40-2.07-fold higher and 1.29-1.38-fold higher separately in roots and shoots of transgenic plants than those in wild-type plants, while the transfer coefficient value kept invariably even decreased. Therefore, PtoEXPA12 was really involved in Cd uptake and accumulation, and led to Cd toxicity of cells. It would be a potentially applicable part in phytoremediation system.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29758311</PMID><DateCompleted><Year>2018</Year><Month>10</Month><Day>23</Day></DateCompleted><DateRevised><Year>2018</Year><Month>10</Month><Day>23</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-0003</ISSN><JournalIssue CitedMedium="Internet"><Volume>116</Volume><PubDate><Year>2018</Year><Month>Sep</Month></PubDate></JournalIssue><Title>International journal of biological macromolecules</Title><ISOAbbreviation>Int J Biol Macromol</ISOAbbreviation></Journal><ArticleTitle>Over-expression of the poplar expansin gene PtoEXPA12 in tobacco plants enhanced cadmium accumulation.</ArticleTitle><Pagination><MedlinePgn>676-682</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0141-8130(18)30177-6</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.ijbiomac.2018.05.053</ELocationID><Abstract><AbstractText>As important cell wall proteins in plants, expansins are involved in a serious of abiotic stress resistance such as drought, heat, salt, even heavy metals. To understand the role of expansins in cadmium (Cd) stress, we analyzed the expression patterns of 36 expansin genes in Populus tomentosa. A Cd-induced expansin gene, PtoEXPA12, was identified, cloned, and transformed into tobacco plants. After treatment with Cd, the transgenic plants showed stronger symptoms of Cd toxicity as to the wild-type tobacco plants. Further physiological tests showed that the transformants had higher relative electrolyte leakage and superoxide dismutase activity, more malondialdehyde and H<sub>2</sub>O<sub>2</sub> content, and lower chlorophyll content in Cd stress. Cd content measurement showed it is 1.40-2.07-fold higher and 1.29-1.38-fold higher separately in roots and shoots of transgenic plants than those in wild-type plants, while the transfer coefficient value kept invariably even decreased. Therefore, PtoEXPA12 was really involved in Cd uptake and accumulation, and led to Cd toxicity of cells. It would be a potentially applicable part in phytoremediation system.</AbstractText><CopyrightInformation>Copyright © 2018. Published by Elsevier B.V.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Hao</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>National Engineering Laboratory of Tree Breeding, Beijing Forestry University, 100083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ding</LastName><ForeName>Yana</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>National Engineering Laboratory of Tree Breeding, Beijing Forestry University, 100083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhi</LastName><ForeName>Junkai</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>National Engineering Laboratory of Tree Breeding, Beijing Forestry University, 100083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Xiaoyu</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>National Engineering Laboratory of Tree Breeding, Beijing Forestry University, 100083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Huabo</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>National Engineering Laboratory of Tree Breeding, Beijing Forestry University, 100083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Jichen</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>National Engineering Laboratory of Tree Breeding, Beijing Forestry University, 100083, China. Electronic address: jcxu282@sina.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>05</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Int J Biol Macromol</MedlineTA><NlmUniqueID>7909578</NlmUniqueID><ISSNLinking>0141-8130</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C095825">expansin protein, plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>00BH33GNGH</RegistryNumber><NameOfSubstance UI="D002104">Cadmium</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002104" MajorTopicYN="N">Cadmium</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="Y">Plant Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="Y">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="Y">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Cadmium accumulation</Keyword><Keyword MajorTopicYN="N">Expansin</Keyword><Keyword MajorTopicYN="N">Phytoremediation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>01</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>05</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>05</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>5</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>10</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>5</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29758311</ArticleId><ArticleId IdType="pii">S0141-8130(18)30177-6</ArticleId><ArticleId IdType="doi">10.1016/j.ijbiomac.2018.05.053</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhang, Hao" sort="Zhang, Hao" uniqKey="Zhang H" first="Hao" last="Zhang">Hao Zhang</name></noRegion><name sortKey="Ding, Yana" sort="Ding, Yana" uniqKey="Ding Y" first="Yana" last="Ding">Yana Ding</name><name sortKey="Li, Xiaoyu" sort="Li, Xiaoyu" uniqKey="Li X" first="Xiaoyu" last="Li">Xiaoyu Li</name><name sortKey="Liu, Huabo" sort="Liu, Huabo" uniqKey="Liu H" first="Huabo" last="Liu">Huabo Liu</name><name sortKey="Xu, Jichen" sort="Xu, Jichen" uniqKey="Xu J" first="Jichen" last="Xu">Jichen Xu</name><name sortKey="Zhi, Junkai" sort="Zhi, Junkai" uniqKey="Zhi J" first="Junkai" last="Zhi">Junkai Zhi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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